Rab27b Is a Potential Indicator for Lymph Node Metastasis and Unfavorable Prognosis in Lung Adenocarcinoma

Figure 2

One-step quantitative real-time polymerase chain reaction (qPCR) test and Western blot analysis were performed to detect the mRNA and protein expression of Rab27b in lung adenocarcinoma (LUAD) cell lines and tissue samples. A549, H1299, and H1975 are three LUAD cell lines. HBE is a noncancerous human bronchial epithelial cell line. (a, b) qPCR and Western blot analyses demonstrated that Rab27b expression was significantly higher in LUAD cell lines than in a normal HBE cell line. (c, d) The expression of Rab27b was also elevated in LUAD tissues compared with noncancerous tissues. Ca: LUAD tissue samples; N: corresponding noncancer tissue samples.

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