Microtubule-Associated Protein 4 Is a Prognostic Factor and Promotes Tumor Progression in Lung Adenocarcinoma

Figure 3

MAP4 expression was repressed by MAP4 siRNA in lung cancer cells. A549 and H1299 cells were transfected with siRNA against MAP4 and negative control sequence (siNC). Expression levels of MAP4 protein in siMAP4-transfected cells were significantly lower than those in siNC-transfected cells by Western blot analysis (a). Densitometry analysis of MAP4 expression in siMAP4-transfected A549 and H1299 cells (b). β-actin levels served as a loading control. The ratio of MAP4 expression to β-actin was calculated and represented as mean + SD relative to the control (, Student’s t-test).

(a)

(b)