Patterns of TIGIT Expression in Lymphatic Tissue, Inflammation, and Cancer
Table 1
List of the used antibodies, antigen retrieval (AR), dilutions, and Opal dyes.
Antibody
Target
Bright field
Fluorescence
AR
Dilution
AR
Dilution
Order1
Dye
DAKO #IR503
CD3
pH 9
1 : 1
pH 9
1 : 1
1st
Opal 520
DAKO #IR649
CD4
pH 9
1 : 1
pH 9
1 : 1
1st
Opal 520
DAKO #IR623
CD8
pH 9
1 : 1
pH 9
1 : 1
1st
Opal 520
BioLegend #320102
FOXP3
pH 9
1 : 50
pH 9
1 : 50
1st
Opal 520
DAKO #IR604
CD20
pH 9
1 : 1
pH 9
1 : 10
1st
Opal 520
DAKO #IR613
CD68
pH 6
1 : 1
pH 6
1 : 1
1st
Opal 520
DAKO #IR628
CD56
pH 9
1 : 1
pH 9
1 : 1
1st
Opal 520
Abcam#ab52632
CD11c
pH 9
1 : 450
pH 9
1 : 450
1st
Opal 520
Dianova #DIA-TG1
TIGIT
pH 7.8
1 : 70
pH 9
1 : 150
2nd
Opal 570
Abcam #ab52587
PD-1
pH 6
1 : 50
pH 6
1 : 50
3rd
Opal 690
AR: antigen retrieval; 1order refers to the sequence of antibodies in multiplex fluorescence immunohistochemistry experiments; antibody was used at third position when stained in combination with CD3, CD4; with Opal 690 dye.