Research Article

Patterns of TIGIT Expression in Lymphatic Tissue, Inflammation, and Cancer

Table 1

List of the used antibodies, antigen retrieval (AR), dilutions, and Opal dyes.

AntibodyTargetBright fieldFluorescence
ARDilutionARDilutionOrder1Dye

DAKO #IR503CD3pH 91 : 1pH 91 : 11stOpal 520
DAKO #IR649CD4pH 91 : 1pH 91 : 11stOpal 520
DAKO #IR623CD8pH 91 : 1pH 91 : 11stOpal 520
BioLegend #320102FOXP3pH 91 : 50pH 91 : 501stOpal 520
DAKO #IR604CD20pH 91 : 1pH 91 : 101stOpal 520
DAKO #IR613CD68pH 61 : 1pH 61 : 11stOpal 520
DAKO #IR628CD56pH 91 : 1pH 91 : 11stOpal 520
Abcam#ab52632CD11cpH 91 : 450pH 91 : 4501stOpal 520
Dianova #DIA-TG1TIGITpH 7.81 : 70pH 91 : 1502ndOpal 570
Abcam #ab52587PD-1pH 61 : 50pH 61 : 503rdOpal 690

AR: antigen retrieval; 1order refers to the sequence of antibodies in multiplex fluorescence immunohistochemistry experiments; antibody was used at third position when stained in combination with CD3, CD4; with Opal 690 dye.