Disease Markers / 2019 / Article / Fig 2

Research Article

Presence of Circulating miR-145, miR-155, and miR-382 in Exosomes Isolated from Serum of Breast Cancer Patients and Healthy Donors

Figure 2

Characterization of exosomes by tracking analysis (NTA) and Western blot. (a) The size of the exosomes (nm) isolated from the serum of BC patients and healthy donor was determined through NTA using a NanoSight NS300 instrument (NanoSight Ltd., Amesbury, UK). All measurements were done by triplicate, and values are presented as . Statistical analysis was conducted by the Student -test. ; ; [5 healthy donors, 5 BC patients per BC stage (I-IV)]. (b) Immunobloting of the exosomal markers CD63, Alix, Tsg, CD9, and CD81. A concentration of 30 μg of proteins was used. Enhanced chemoluminescence detection kit (GE Healthcare, Piscataway, NJ) was used to visualize protein bands. Three representative samples from healthy donors and BC patients are shown.

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