Long-Chain Fatty Acids and Inflammatory Markers Coaccumulate in the Skeletal Muscle of Sarcopenic Old Rats

Figure 2

Schematic of the SPE procedure for GC-MS analysis of FAMEs from rat tissue or plasma. Total lipid fractions from plasma or tissue were separated on 2 SPE columns to isolate a fraction of FFAs or another fraction containing CEs, TAGs, and GPLs, respectively. For each fraction, a second SPE column was used to purify the individual FFA, CE, TAG, or GPL fractions for further GC-MS analysis.