Promoter Hypomethylation Is Responsible for Upregulated Expression of HAI-1 in Hepatocellular Carcinoma

Figure 4

Analysis of the expression level and promoter methylation status of HAI-1 in primary HCC tissues. (a, b) Immunohistochemical staining of HAI-1 in HCC. HAI-1 was expressed in the cell membrane and cytoplasm. (a) Representative images of microscopic immunohistochemical staining for HAI-1 in HCC. Magnification ×400; . (b) Representative photomicrographs of immunohistochemical staining of HAI-1 in adjacent nontumor surrounding HCC liver tissue were negative for HAI-1 expression. An arrow indicated as bile canaliculi. The biliary epithelial cells were positive for HAI-1. (c) Representative photomicrographs of HCC tissues and normal liver tissues illustrating expression levels of HAI-1 immunohistochemical staining (0, 1, 2, and 3). (d) Representative analysis of HAI-1 methylation in primary tumors (T) and paired nontumor tissues (N) by MSP. . (e) Relative methylation level of HAI-1 by qMSP in highly, moderately, and poorly differentiated HCC tumors. ; , ; .

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