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Disease Markers
Volume 2019, Article ID 9429323, 8 pages
https://doi.org/10.1155/2019/9429323
Research Article

Corin Is Downregulated in Renal Ischemia/Reperfusion Injury and Is Associated with Delayed Graft Function after Kidney Transplantation

1Department of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing, China
2Department of Clinical Laboratory, Peking University People’s Hospital, Beijing, China

Correspondence should be addressed to Ye Tian; moc.621@oainimiyuoy and Wei Qiu; moc.361@816iewuiq

Received 20 September 2018; Revised 31 October 2018; Accepted 8 November 2018; Published 14 January 2019

Academic Editor: Alexandra Scholze

Copyright © 2019 Xinyi Hu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Renal ischemia/reperfusion (IR) injury is one of the most important risk factors for the occurrence of delayed graft function (DGF) after kidney transplantation; however, its mechanism remains not fully understood. In the present study, we screened differentially expressed genes in a murine model of renal IR injury by using high-throughput assays. We identified Corin as one of the most significantly downregulated genes among 2218 differentially expressed genes (≥2-fold, ). By using a real-time qPCR assay, we observed that the expression of renal Corin in IR-injured mice was reduced to 11.5% of the sham-operated mice and that the protein level of renal Corin in IR-injured mice was also downregulated. Interestingly, renal IR injury in mice induced the downregulation of Corin in heart tissues, suggesting that the overall synthesis of Corin may be suppressed. We recruited 11 recipients complicated with DGF and 16 without DGF, and plasma Corin concentrations were determined by ELISA. We observed that the plasma Corin levels were indeed reduced in recipients complicated with DGF (0.98 vs. 1.95 ng/ml, ). These findings demonstrate that Corin may be a potential biomarker of DGF after kidney transplantation and may participate in the regulation of renal IR injury.