Molecular Markers for Detecting Schistosoma Species by Loop-Mediated Isothermal Amplification

Figure 4

LAMP assay for amplifying S. bovis NADH-1. (a). Specificity assessment. Only NADH-1 was amplified using S. bovis DNA. Sm: S. mansoni DNA; Sh: S. haematobium DNA; Sj: S. japonicum DNA; Si: S. intercalatum DNA; Sb: S. bovis DNA; N: negative controls (ultrapure water, no DNA). (b). Sensitivity assessment. Sb: S. bovis genomic DNA (10 ng/μL); lanes 10^-1-10^-9, 10-fold serially dilutions.

(a)

(b)