MicroRNA-146-5p Promotes Pulmonary Artery Endothelial Cell Proliferation under Hypoxic Conditions through Regulating USP3

Figure 5

Overexpression of USP3 attenuates the effect of overexpression of miR-146-5p on the proliferative capacity of HPAEC cells after 48 h of hypoxic treatment. (a) qRT-PCR was used to detect the mRNA expression level of miR-146-5p in HPAEC cells after cotransfection of miR-146-5p and USP3 overexpression vectors after 48 h of hypoxia. (b) CCK-8 detected the proliferative capacity of HPAEC cells after cotransfection of miR-146-5p and USP3 overexpression vectors after 48 h of hypoxia. (c) The EdU assay detected the number of positive proliferating cells in HPAECs after cotransfection of miR-146-5p and USP3 overexpression vectors after 48 h of hypoxia. (d) Western blotting was used to detect the protein expression level of USP3 in HPAEC cells after cotransfection of miR-146-5p and USP3 overexpression vectors after 48 h of hypoxia. Data were , and indicates a significant difference ().

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