Research Article
CircLDLR Promotes Papillary Thyroid Carcinoma Tumorigenicity by Regulating miR-637/LMO4 Axis
Figure 4
miR-637 interference reverses the antitumor effects induced by si-circLDLR in PTC cells. TPC-1 and IHH-4 cells were transfected with si-NC, si-circLDLR, si-circLDLR + anti-NC, or si-circLDLR + anti-miR-637. (a) qRT-PCR analysis of miR-637 expression after transfection. (b, c) The proliferation analysis of TPC-1 and IHH-4 cells using CCK-8 assay and colony formation assay. (d) Apoptosis analysis of TPC-1 and IHH-4 cells using flow cytometry. (e, f) Analysis of TPC-1 and IHH-4 cell migration and invasion with transwell assay and wound healing assay. (g, h) Levels detection of C-caspase3, MMP2, and MMP9 protein levels using western blot. .
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