Research Article

CircLDLR Promotes Papillary Thyroid Carcinoma Tumorigenicity by Regulating miR-637/LMO4 Axis

Figure 6

MiR-637 suppresses cell malignant phenotypes in PTC cells by targeting LMO4. TPC-1 and IHH-4 cells were transfected with NC, miR-637, miR-637 + vector, or miR-637 + LMO4. (a) Western blot analysis of LMO4 expression in TPC-1 and IHH-4 cells after transfection. (b, c) The proliferation analysis of TPC-1 and IHH-4 cells using CCK-8 assay and colony formation assay. (d) Flow cytometry analysis of apoptosis of TPC-1 and IHH-4 cells. (e, f) The analysis of invasion and migration abilities of TPC-1 and IHH-4 cells using transwell assay and wound healing assay. (g, h) Western blot analysis of C-caspase3, MMP2, and MMP9 protein levels. .
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