Effects of Long Noncoding RNA HOTAIR Targeting miR-138 on Inflammatory Response and Oxidative Stress in Rat Cardiomyocytes Induced by Hypoxia and Reoxygenation

Figure 3

Effect of silenced miR-138 on H9C2 cells. (a) The luciferase reporter assay was used to detect the targeting relationship between miR-138 and HOTAIR. (“” indicates statistical difference from the HOTAIR wt+miR-138 NC group .) (b) qRT-PCR was used to detect the expression of IL-1β. (c) qRT-PCR was used to detect the expression of IL-6. (d) qRT-PCR was used to detect the expression of TNF-α. (e) WB was used to detect the expression of IL-1β, IL-6, and TNF-α. (f) ELISA was used to detect the content of IL-1β. (g) ELISA was used to detect the content of IL-6. (h) ELISA was used to detect the content of TNF-α. (i) ELISA was used to detect the content of MDA. (j) ELISA was used to detect the activity of SOD. (k) Immunofluorescence staining was used to detect the expression of SOD1 (magnification: 400x). (“” indicates statistical difference from the H/R group, , and “#” indicates statistical difference from the H/R+si-HOTAIR group, ).

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