Fibronectin 1: A Potential Biomarker for Ovarian Cancer

Figure 4

The FN1 KO cell was used for further confirmation. (a) The mRNA expression of FN1 was tested by real-time PCR among A2780, Caov3, OVCAR3, and Skov3. (b) The cell protein level of FN1 was detected by western blot among A2780, Caov3, OVCAR3, and Skov3. (c) The FN1 level in cell medium was detected by ELISA among A2780, Caov3, OVCAR3, and Skov3. (d) The knockout FN1 mRNA efficiency was detected by real-time PCR among wild-type cell (WT), control cell (vector V2), and the KO cell (V2-sgRNA-FN1). (e) The knockout FN1 protein level efficiency was detected by real-time PCR among wild-type cell (WT), control cell (vector V2), and the KO cell (V2-sgRNA-FN1). (f) Transwell assays were conducted among wild-type cell (WT), control cell (vector V2), and the KO cell (V2-sgRNA-FN1). (g) Quantitative results of the transwell invasion assays. Significant differences were identified using unpaired -test analyses and are indicated by asterisks; and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)