Cancer-Associated Fibroblasts Regulate Bladder Cancer Invasion and Metabolic Phenotypes through Autophagy

Figure 1

Downregulation of Atg5 in hFF cells. (a) Schematic representation of a Transwell coculture system: hFF cells were seeded in the upper chamber of a 6-well Transwell and T24 cells were in the bottom chamber (pore size: 0.4 μm). After 48 hrs, T24 cells were trypsinized acquired for further experiments; the effects of Atg5 siRNA on hFF cells were detected by RT-PCR (b) and Western blot (c, d). Because of the high efficiency in decreasing Atg5 expression, siAtg5-3 was chosen for further experiments.

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