Research Article
Aberrant Hypermethylation-Mediated Suppression of PYCARD Is Extremely Frequent in Prostate Cancer with Gleason Score ≥ 7
Figure 3
PYCARD reexpression induces apoptosis in LNCaP cells. (a) Immunoblotting analysis of PYCARD protein using anti-PYCARD antibody in LNCaP-derived cell lines. HMV-II is a human melanoma cell line used as a positive control. ACTB was used as an endogenous control. (b) Immunofluorescence staining of PYCARD (630x magnification). (c) Effects of PYCARD induction on TR15_PYCARD cell proliferation. and denote and , respectively. Light microscopic appearances (40x magnification) of these cells are shown below. (d) Results of flow cytometry.
(a) |
(b) |
(c) |
(d) |