Disease Markers / 2021 / Article / Fig 3

Research Article

The Role, Function, and Mechanism of Long Intergenic Noncoding RNA1184 (linc01184) in Colorectal Cancer

Figure 3

linc01184 directly targeted miR-331 and acted as ceRNA of miR-331 in CRC cells. (a) The prediction of the binding region between linc01184 and miR-331; (b) the sequence of wild-type (WT) and mutant (MUT) linc01184 luciferase reporters; (c) dual-luciferase reporter assay revealed that linc01184 directly targeted miR-331, vs. luci-linc01184-MUT or negative control (NC) mimic; (d) the analysis of enrichment between linc01184 and miR-331 probes using the RNA pull-down assay, vs. control probe or miR-331 mutant (MUT) probe; (e) linc01184 expression in transfected HCT-116 cells, vs. empty vector, # vs. 0.5 μg/mL pCDNA-linc01184; (f) miR-331 expression in transfected HCT-116 cell, vs. empty vector or scramble control.