Research Article

Human Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying MicroRNA-29a Improves Ovarian Function of Mice with Primary Ovarian Insufficiency by Targeting HMG-Box Transcription Factor/Wnt/β-Catenin Signaling

Figure 1

Identification of UCMSCs and characterization of exosomes derived from UCMSCs. (a) The morphology of human UCMSCs at the fourth passage, the Alizarin Red staining of osteogenic identification, and the Oil Red O staining of adiopogenic differentiation. (b, c) Positive expression of CD29, CD44, and CD90 and negative expression of CD14, CD34, and HLA-D-PE were detected by flow cytometry. (d) Western blotting was performed to measure the protein level of positive exosome marker (CD63) and negative exosome marker (calnexin).