Research Article

Human Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying MicroRNA-29a Improves Ovarian Function of Mice with Primary Ovarian Insufficiency by Targeting HMG-Box Transcription Factor/Wnt/β-Catenin Signaling

Figure 4

miR-29a targets HBP1 3UTR. (a) miRDB of downstream target prediction () (http://mirdb.org/). (b) RT-qPCR was carried out to measure the levels of candidate mRNAs in the context of miR-29a upregulation. (c) The protein level of HBP1 after miR-29a upregulation was determined by western blotting. (d) TargetScan of binding site prediction (http://www.targetscan.org/vert_80/). (e) The binding sites between miR-29a and HBP1 among species. (f) Luciferase reporter assays were conducted to verify the binding relationship of miR-29a and HBP1. (g) The relationship between miR-29a and HBP1 was tested by RNA pull-down assays. .
(a)
(b)
(c)
(d)
(e)
(f)
(g)