Overexpression of Activating Transcription Factor-2 (ATF-2) Activates Wnt/Ca²⁺ Signaling Pathways and Promotes Proliferation and Invasion in Non-Small-Cell Lung Cancer

Figure 4

ATF-2 was knocked down in NSCLC cells, and the Wnt pathway agonist was added to detect pathway activity and cell proliferation activity. (a, b) qPCR and WB were used to detect the activity of downstream factors and determine the efficiency of agonists in the pathway. (c) WB was used to detect the expression of pathway proteins in different treatment groups. (d) CCK8 was used to detect cell proliferation. (e) Transwell tested the invasion ability of cells. Compared to the control group, ; compared to the si-ATF-2 group, ^#; the difference was statistically significant.

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