Research Article

Downregulation of MCF2L Promoted the Ferroptosis of Hepatocellular Carcinoma Cells through PI3K/mTOR Pathway in a RhoA/Rac1 Dependent Manner

Figure 5

MCF2L regulates PI3K/mTOR pathway in a Rac1/RhoA-dependent manner. (a) Downregulation of MCF2L significantly inhibits the phosphorylation levels of PI3K (p-PI3K), AKT (p-AKT), mTOR (p-mTOR), and the levels of mTOR pathway mediators, p-S6K1 and p-4E-BP1. (b) Downregulation of MCF2L inhibits the activity of RhoA and Rac1. (c) MTT and (d) colony formation assay showed that inhibitors (NSC23766 and RhoGDIs) can significantly reversed the viability of HCC cells that promoted by overexpression of MCF2L compared with vehicle. (e) The relative ROS levels of HCC cells transfected with MCF2L plasmid treated using inhibitors (NSC23766 and RhoGDIs) or vehicle. (f) The relative MDA levels of HCC cells transfected with MCF2L treated using inhibitors (NSC23766 and RhoGDIs) or vehicle. .
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