Cinnamaldehyde Downregulation of Sept9 Inhibits Glioma Progression through Suppressing Hif-1α via the Pi3k/Akt Signaling Pathway

Figure 3

The effects of CA on cell apoptosis and invasive ability of U87 and U251 cells. (a) The cells were inoculated in 6-well plates and treated with CA (4 μg/ml and 8 μg/ml) for 24 hours. Annexin V-FITC/PI apoptosis was detected and photographed under a fluorescence microscope. (b) Statistical results of fluorescence microscope images from the apoptosis experiment. (c) CA induces apoptosis through the regulation of apoptosis-related genes. U87 and U251 cells were treated with 0, 4, and 8 μg/mL CA for 24 hours. Western blot was used for detecting the expressions of Bcl-2 and Bax, and β-actin was the control. (d) The expression levels of Bax and Bcl-2 were measured. The effect of CA was then evaluated using Bax/Bcl-2 ratio. (e) Following treatment with different concentrations (4 μg/ml and 8 μg/ml) of CA, the cells migrated through pores of 8 μm in diameter to the lower lumen within 24 hours, where images were captured using a light microscope (magnification 400x). The white dots are the 8 μm diameter pores of the transwell chamber. (f) Statistical results of invasion experiment. (g) CA suppresses the expressions of MMP-2 and MMP-9 while increasing the expression of E-cadherin. U87 and U251 cells were treated with CA (4 and 8 μg/ml) for 24 hours. The results of a minimum of three independent trials are presented as . and compared to the control group.

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