Perisciatic Nerve Dexmedetomidine Alleviates Spinal Oxidative Stress and Improves Peripheral Mitochondrial Dynamic Equilibrium in a Neuropathic Pain Mouse Model in an AMPK-Dependent Manner

Figure 2

Impact of local injection of DEX around the sciatic nerve on oxidative phosphorylation and antioxidant stress ability of the mitochondria in the injured nerve of CCI mice. DEX increased the expression level of mitochondrial ETC complexes (a) I, (b) II, (c) III, and (d) IV in the injured nerve of CCI mice, and its effect could be partly inhibited by the AMPK inhibitor, compound C. DEX increased the (e) ATP and (f) SOD content in the injured nerve of CCI mice, and its effect was suppressive in part through the compound C (CC) injection. (g) DEX promoted GSH production in the injured nerve of CCI mice, and its effect was suppressive in part through the CC injection. (h) DEX inhibited GSH production in the injured nerve of CCI mice, and its effect was suppressive in part through the CC injection. Data presented are () from triplicate separate experiments. and against negative control; ^## and ^### against CCI group; ^$, ^$$, and ^$$$ CCI+DEX against CCI+DEX+CC groups.

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