Perisciatic Nerve Dexmedetomidine Alleviates Spinal Oxidative Stress and Improves Peripheral Mitochondrial Dynamic Equilibrium in a Neuropathic Pain Mouse Model in an AMPK-Dependent Manner

Figure 3

Impact of DEX on the pAMPK expression in the injured nerve of CCI mice. (a) Immunofluorescence localization showed that the local injection of DEX around the sciatic nerve elevated the pAMPK-positive cell counts prominently in the distal nerve after CCI. Micrographic magnification: ×100, scale bars: 50 μm. (b) Histogram depicting an intergroup comparison of immunohistochemical scores. (c) Western blot was adopted to detect the expression of PAMPK in the distal nerve after CCI. (d) Densitometric quantification of pAMPK/GAPDH. Data are expressed as () and represent three independent experiments. versus negative control group; ^# and ^### versus CCI group; ^$ and ^$$$ CCI+DEX versus CCI+DEX+CC group.

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