Effects of Escin on Oxidative Stress and Apoptosis of H9c2 Cells Induced by H₂O₂

Figure 2

Escin inhibited oxidative stress in H9c2 cells. (a) The expression of SOD1 and SOD2 in the H₂O₂ treatment group decreased significantly, and the expression of SOD1 and SOD2 increased in the H₂O₂+escin group. (b) Statistical results of protein level of SOD1 ( vs. control; ^# vs. H₂O₂, ). (c) Statistical results of protein level of SOD2 ( vs. control; ^# vs. H₂O₂, ). (d) The expression of SOD1 mRNA in the H₂O₂ treatment group decreased significantly, and the expression increased in the H₂O₂+escin group ( vs. control; ^# vs. H₂O₂, ). (e) The expression of SOD2 mRNA in the H₂O₂ treatment group decreased significantly, and the expression increased in the H₂O₂+escin group ( vs. control; ^# vs. H₂O₂, ). (f) SOD activity assay showed that H₂O₂ can significantly reduce SOD levels, while escin can reverse SOD levels ( vs. control; ^# vs. H₂O₂, ). (g) The expression of ROS increased in the H₂O₂ treatment group but decreased significantly in the H₂O₂+escin group ( vs. control; ^# vs. H₂O₂, ).

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