Effects of Escin on Oxidative Stress and Apoptosis of H9c2 Cells Induced by H₂O₂

Figure 3

Escin inhibited apoptosis of H9c2 cells. (a) The expression of Bcl-2 in the H₂O₂ treatment group decreased significantly but increased in the H₂O₂+escin group. Bax expression was opposite to Bcl-2. (b) Statistical results of protein level of Bcl-2 ( vs. control; ^# vs. H₂O₂, ). (c) Statistical results of expression of Bax ( vs. control; ^# vs. H₂O₂, ). (d) The expression of Bcl-2 mRNA was consistent with the Bcl-2 protein ( vs. control; ^# vs. H₂O₂, ). (e) The expression of Bax mRNA was consistent with the Bax protein ( vs. control; ^# vs. H₂O₂, ). (f) TUNEL staining showed that escin can obviously reduce the increase of H9c2 cell apoptosis caused by H₂O₂ (magnification: ×400). (g) The apoptotic rate of the H₂O₂ treatment group increased and decreased in the H₂O₂+escin group. (h) Statistical results of apoptotic rate of H9c2 cells ( vs. control; ^# vs. H₂O₂, ).

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