Inhibition of DEK Enhances Doxorubicin-Induced Apoptosis and Cell Cycle Arrest in T-Cell Acute Lymphoblastic Leukemia Cells

Figure 2

DEK silencing efficiently suppresses cell proliferation. (a) DEK mRNA levels relative to GAPDH levels in Jurkat cells infected with three different lentiviruses (SCR, shDEK-1, and shDEK-2) as detected by RT-PCR. (b) Western blotting was conducted to confirm that shDEK efficiently knocked down DEK protein expression in Jurkat cells. (c) Cell proliferation assay. Cell numbers were counted at 0, 2, 4, and 6 days after transduction. Data are expressed as the . vs. SCR; . (d) Colony formation assay. The number of colonies formed by Jurkat cells was counted at 14 days after transduction. (e) Representative images of colonies formed by DEK KD Jurkat cells after 14 days. and .

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