Research Article

Systematic Pan-Cancer Analysis and Experimental Verification Identify FOXA1 as an Immunological and Prognostic Biomarker in Epithelial Ovarian Cancer

Figure 7

Effects of FOXA1 silencing on OVCAR-3 cell proliferation and invasion. (a) The proliferation was measured by CCK8 assay at 24, 48, and 72 h, respectively. The results were obtained from the 6 replicates in each group and presented as average . (b) The invasiveness of OVCAR-3 cells was detected by the Transwell migration assay. Representative images were selected (magnification ×100). (c) The number of invaded cells were quantified and obtained from three independent experiments. (d) Protein levels of E-cadherin, vimentin, and Snail were measured by western blot after 48 h following siRNA transfection. (e) Quantifications of representative blots were demonstrated in bar graphs. Protein levels of E-cadherin, vimentin, and Snail in the FOXA1-silenced group (siRNA-FOXA1) were compared to those in the control group (siRNA-NC) and presented in the form of . (f, g) Representative immunofluorescence staining for E-cadherin (f) and vimentin (g) in the siRNA-NC- or siRNA-FOXA1-transfected OVCAR3 cells (original magnification ×630).