circRNA circ_0055724 Inhibits Trophoblastic Cell Line HTR-8/SVneo’s Invasive and Migratory Abilities via the miR-136/N-Cadherin Axis

Figure 5

circ_0055724 mediated trophoblast invasion and migration via the miR-136/N-cadherin axis. (a) The WB method was used to detect the low level of N-cadherin in HTR-8/SVneo cells. (b) The CCK-8 method was used to detect the light absorption values of HTR-8/SVneo cells at 450 nm at 0 h, 24 h, 48 h, 72 h, and 96 h in different groups. (c) The clonal formation experiment was conducted to count the number of colonies and thereby estimate the colony forming capacity of the HTR-8/SVneo cells. (d) Cell migration ability of HTR-8/SVneo cells in different groups was detected by Transwell assay. (e) Invasion ability of HTR-8/SVneo cells in different groups is represented and determined by Transwell assay. ; .

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