TY - JOUR A2 - Jinhui, Liu AU - Meng, Shuhan AU - Wen, Dan AU - Xiao, Jingge AU - Zhang, Qianyue AU - Fang, Weizhou AU - Xue, Xiao AU - Hu, Tu AU - Xia, Xiaobo PY - 2022 DA - 2022/01/29 TI - Age of Rats Affects the Degree of Retinal Neuroinflammatory Response Induced by High Acute Intraocular Pressure SP - 9404977 VL - 2022 AB - Purpose. To investigate whether retinal neuroinflammatory response was affected by aging in a rat model of acute glaucoma. Methods. Young adult and aged rats were randomly assigned into normal control, 45 mmHg, 60 mmHg, and 90 mmHg groups. Intraocular pressure (IOP) of rats was acutely elevated to 45 mmHg, 60 mmHg, and 90 mmHg, respectively. Three days after high IOP treatment, loss of retinal ganglion cells (RGCs), formation of proinflammatory microglia/macrophages and neurotoxic astrocytes, and deposition of complement C3 in the retina were detected by immunofluorescence. ELISA was used to assess the protein levels of proinflammatory cytokines TNF and IL-1β in the retina. Results. Compared with young adult retinae, (1) loss of RGCs was more severe in aged retinae under the same IOP treatment, (2) microglia/macrophages were more prone to adopt proinflammatory phenotype in aged retinae in response to elevated IOP, (3) high IOP treatment induced astrogliosis, formation of neurotoxic astrocytes, and deposition of complement C3 more easily in aged retinae, and (4) aged retinae induced higher levels of proinflammatory cytokines TNF and IL-1β under the same IOP treatment. Conclusion. Our data indicated that aging affects the degree of retinal neuroinflammatory response initiated by ocular hypertension, which may contribute to the age-related susceptibility of RGCs to elevated IOP. SN - 0278-0240 UR - https://doi.org/10.1155/2022/9404977 DO - 10.1155/2022/9404977 JF - Disease Markers PB - Hindawi KW - ER -