Age of Rats Affects the Degree of Retinal Neuroinflammatory Response Induced by High Acute Intraocular Pressure

Figure 2

Microglia/macrophages were more prone to exhibit proinflammatory phenotype in aged retinae than that of young adult retinae after IOP treatment. Significant increase in CD68⁺IBA1⁺ (d, q) and MHC-II⁺IBA1⁺ cells (l, r) was observed in young adult retinae at IOP of 90 mmHg. Aged retinae showed dramatic increase in CD68⁺IBA1⁺ (g–h, q) and MHC-II⁺IBA1⁺ cells (o–p, r) since IOP of 60 mmHg. Compared with the young adult retinae, aged retinae induced more CD68⁺IBA1⁺ (q) and MHC-II⁺IBA1⁺ cells (r) at 60 and 90 mmHg 3 days after IOP treatment (GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer. , versus age-matched control; versus matched part of young adult retina at the same IOP treatment. Scale bar: 50 μm).