Research Article

Characterization of Epithelial-Mesenchymal Transition Identifies a Gene Signature for Predicting Clinical Outcomes and Therapeutic Responses in Bladder Cancer

Figure 3

Construction of EMT-related gene signature. Volcano plots show DEGs (a) between BC and adjacent normal samples, and prognostic genes (b) calculated by the univariable Cox regression. Red dots are upregulated genes or risk genes, and blue dots present downregulated genes or protective genes. (c) Venn diagram shows 13 intersected genes between DEGs and prognostic genes. (d) The correlation of 13 EMT-related genes in BC. Upregulated genes and downregulated genes are represented with grey and red colors. Risk genes are described in blue and protective genes are colored in green. The values of the Cox regression test for 13 genes are represented by the size of circles. Correlation analysis is performed on 13 genes, depicted by the connecting lines between each gene. Red and blue lines present positive and negative correlations. (e) Variable importance plot based on random forest survival analysis for 13 genes. Blue color indicates predictive variables, whereas red color represents nonpredictive variables. (f) Forest plot based on univariable Cox regression analysis shows that four genes in this signature are all significantly associated with overall survival. (g) The coefficient of each gene in the gene signature is depicted by bar plots. DEGs: differentially expressed genes.