Table of Contents
Dataset Papers in Biology
Volume 2013 (2013), Article ID 949637, 3 pages
http://dx.doi.org/10.7167/2013/949637
Dataset Paper

Evaluating Wheat Microsatellite Markers for the Use in Genetic Analysis of Thinopyrum, Dasypyrum, and Pseudoroegneria Species

Centre for Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Timiryazevskaya Street 49, Moscow 127550, Russia

Received 25 May 2012; Accepted 18 July 2012

Academic Editors: M. Chen, S. A. Mohammadi, and G. Sun

This dataset has been dedicated to the public domain using the CC0 waiver.

Dataset http://dx.doi.org/10.7167/2013/949637/dataset

Dataset

Dataset Item 1 (Table). Size of the microsatellite fragments (base pair (bp)) amplified on DNA of Th. intermedium, Th. elongatum, P. stipifolia, Th. bessarabicum, Th. ponticum, and D. villosum. A total of 42 SSR markers of wheat were evaluated on their cross-amplification on DNA of Th. intermedium, Th. elongatum, Th. ponticum, Th. bessarabicum, P. stipifolia, and D. villosum. For each accession, two plants were analyzed. The sizes of the peaks are obtained by means of the fragment analysis. The number of the wheat SSR markers that amplified DNA fragments with determined size for Th. ponticum was 33 (78.6%); for Th. intermedium, 28 (66.7%); for Th. elongatum, 24 (57.1%); for Th. bessarabicum, 24 (57.1%); for P. stipifolia, 26 (69.1%); and for D. villosum, 29 (69.0%). Thus, the transferability of the SSR markers to the genomes of the investigated species is comparable to that of oat, rye [8], Aegilops [9, 13], Elymus, and Pseudoroegneria [10]. Twenty-four primer pairs of wheat SSR markers were successfully amplified from all investigated species. The absence of amplification may result from either null allele in a locus or significant differences between wheat and studied species in the flanking regions where primers should anneal [14]. The transferred SSRs can be linked to useful genes or QTLs as among selected markers there are some previously used for the mapping and detection of resistance genes of Th. intermedium (Xwmc221, Xwmc121, and Xcfd68) [15] and Th. elongatum (Xgwm325, Xgwm179, and Xgwm335) [16, 17] and others are linked to QTLs of valuable traits on different wheat chromosomes [18]. The size shown in the table is the sum of the real size of a fragment and 25 bp of M13 tail of forward primer. The localization on wheat chromosomes is shown according to Graingenes 2.0 database [10]. If no peaks are detected, they are shown as “0” size. Where the variants between plant genotypes are observed, they are shown through slash “/”. If a group of peaks is found for one plant and it differs from the group of another plant, the sizes of peaks are shown in brackets. Cases when it is difficult to determine major peaks among multiple peaks are designated “ND” (not determined).

  • Column 1: SSR Marker
  • Column 2: Localization in Wheat Chromosome
  • Column 3: Size of the Amplified Fragments of Th. intermedium
  • Column 4: Size of the Amplified Fragments of Th. elongatum
  • Column 5: Size of the Amplified Fragments of P. stipifolia
  • Column 6: Size of the Amplified Fragments of Th. bessarabicum
  • Column 7: Size of the Amplified Fragments of Th. ponticum
  • Column 8: Size of the Amplified Fragments of D. villosum