Table of Contents
Dataset Papers in Science
Volume 2014 (2014), Article ID 807806, 11 pages
http://dx.doi.org/10.1155/2014/807806
Dataset Paper

Subtracted Transcriptome Profile of Tiger Shrimp (Penaeus monodon) That Survived WSSV Challenge

1Genetic Fingerprinting Laboratory, National Fisheries Research and Development Institute, Corporate 101 Building, Mother Ignacia Avenue, 1103 Quezon City, Philippines
2Department of Biological Science, College of Science/Research Center for the Natural Sciences, Thomas Aquinas Research Center, University of Santo Tomas, España Boulevard, 1008 Manila, Philippines
3Aquaculture Department, Southeast Asian Fisheries Development Center (SEAFDEC AQD), Tigbauan Iloilo, Philippines

Received 15 July 2013; Accepted 5 September 2013; Published 8 May 2014

Academic Editors: A. Hannoufa, Y. Okazaki, and B. Padhukasahasram

Copyright © 2014 Benedict A. Maralit et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

There is increased interest in the development of virus-resistant or improved shrimp stock because production is currently hindered by outbreaks and limited understanding of shrimp defense. Recent advancement now allows for high-throughput molecular studies on shrimp immunity. We used next-generation sequencing (NGS) coupled with suppression subtractive hybridization (SSH) to generate a transcriptome database of genes from tiger shrimp that survived White spot syndrome virus (WSSV) challenge. A total of 9,597 unique sequences were uploaded to NCBI Sequence Read Archive with accession number SRR577080. Sixty-five unique sequences, 6% of the total, were homologous to genes of Penaeus monodon. Genes that were initially related to bacterial infection and environmental stress such as 14-3-3 gene, heat shock protein 90, and calreticulin were also found including a few full-length gene sequences. Initial analysis of the expression of some genes was done. Hemocyanin, ferritin, and fortilin-binding protein exhibited differential expression between survivor and control tiger shrimps. Furthermore, candidate microsatellite markers for brood stock selection were mined and tested. Four trinucleotide and one dinucleotide microsatellites were successfully amplified. The study highlights the advantage of the NGS platform coupled with SSH in terms of gene discovery and marker generation.