Experimental Human Cell and Tissue Models of Pemphigus

Figure 1

Incubation of normal human skin with pemphigus IgG or Fab fragments leads to suprabasal or subcorneal acantholytic blistering. (a) Normal human skin (NHS) before incubation. Incubation of NHS for 24 hours in (b) medium only or in medium with added (c) control Fab fragments or (e) control IgG leads to limited spongiosis of the epidermis. Incubation of NHS in medium with added (d) PF Fab fragments and (f) PF IgG induces a subcorneal split. Incubation of NHS in medium with (g) PV IgG induces suprabasal acantholysis.

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(b)

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(g)