Superficial Dsg2 Expression Limits Epidermal Blister Formation Mediated by Pemphigus Foliaceus Antibodies and Exfoliative Toxins

Figure 3

Dsg1 is maintained at the cell-cell border in ETA-treated epidermis. Formalin-fixed paraffin-embedded skin sections from newborn WT and Tg mice treated with ETA, as described in Figure 2, were immunostained with antibodies AP61, AP498, Ab15, and 27B2. Antibodies AP61, AP498, and Ab15 were raised against the extracellular domain of Dsg1-𝛼, Dsg1-𝛽, and Dsg1-𝛾, respectively [17]. Immunofluorescence shows undisturbed cell-cell border staining of all Dsg1 isoforms in both WT and Tg skins treated with ETA. DAPI (blue) was used as a nuclear stain. Similar results were observed in skin treated with ETA for 18 hours. Arrows demarcate site of blister cleavage.