Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin
Figure 6
Malignant melanoma examined ex vivo, 40 μm depth, 760 nm excitation wavelength. (a) Grey scale image acquired by the DermaInspect. We observe an irregular distribution of pleomorphic cells with different shape and size. (b) FLIM image acquired by the DermaInspect equipped with the Imperial College FLIM system. Melanoma cells contain melanin and appear yellow (short lifetime values). (c) The diagram shows the fluorescence lifetime on the x axis (from 0 to 2000 ps) and the number of pixels corresponding to the fluorescence lifetime on the y axis.
