Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique

Figure 4

“Stretched” cell culture conditions realigned matrix fibres with the horizon. Masson’s trichrome stained slices of tissue were analyzed to determine the plane angle of the matrix fibres. (No fibre was found to have an angle superior to 60 degrees.) Distribution of plane angle of standard, “flipped,” and “stretched” conditions was presented. The bars correspond to the mean also reported with standard deviation on the top of the graph. Triangles, squares, and diamonds represent value of the plane angle of the fibres in the same representative experiment. n.s. is for not statistically significant different compared values and is for statistically significant different compared values.