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Evidence-Based Complementary and Alternative Medicine
Volume 5, Issue 1, Pages 55-59
http://dx.doi.org/10.1093/ecam/nel077
Original Article

Inhibitory Response of Raphanus sativus on Lipid Peroxidation in Albino Rats

Department of Biological Sciences, Faculty of Science, University of Botswana, Private bag 0022, Gaborone, Botswana

Received 20 December 2005; Accepted 25 September 2006

Copyright © 2008 P. Chaturvedi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the present study, inhibitory effect of the methanol extract of Raphanus sativus root on lipid peroxidation has been carried out in normal rats. Graded doses of methanol extract of root of the plant (40, 80 and 120 mg kg−1 body weight) were administered orally for 15 days to experimental treated rats. Distilled water was administered to experimental control rats. At the end of experiment, rats were killed by decapitation after ether anesthesia. Blood and liver were collected to measure thiobarbituric acid reactive substance, reduced glutathione and activity of catalase. Results indicated that the extract of R. sativus root reduced the levels of thiobarbituric acid reactive substance significantly in all experimental treated groups (P < 0.05) as compared to the experimental control group. It also increased the levels of reduced glutathione and increased the activity of catalase. In vitro experiments with the liver of experimental control and experimental treated rats were also carried out against cumene hydroperoxide induced lipid peroxidation. The extract inhibited in vitro cumene hydroperoxide induced lipid peroxidation. R. sativus inhibits lipid peroxidation in vivo and in vitro. It provides protection by strengthening the antioxidants like glutathione and catalase. Inclusion of this plant in every day diet would be beneficial.