Original Article | Open Access
Zhu Shijie, Junji Moriya, Jun’ichi Yamakawa, Rui Chen, Takashi Takahashi, Hiroyuki Sumino, Takeshi Nakahashi, Kunimitsu Iwai, Shigeto Morimoto, Nobuo Yamaguchi, Tsugiyasu Kanda, "Mao-to Prolongs the Survival of and Reduces TNF-α Expression in Mice with Viral Myocarditis", Evidence-Based Complementary and Alternative Medicine, vol. 7, Article ID 529290, 9 pages, 2010. https://doi.org/10.1093/ecam/nen010
Mao-to Prolongs the Survival of and Reduces TNF-α Expression in Mice with Viral Myocarditis
Goal of this study was to evaluate effects of Mao-to on development of myocarditis induced by encephalomyocarditis (EMC) virus in mice. Mice were randomly divided into five groups. Group N included uninfected controls (n = 18), while group A, B and C underwent intraperitoneal injection of EMC virus. Group A was administered oral saline from day 0 to day 4. Group B was administered oral Mao-to (500 mg−1 kg−1 day−1) from day 0 to day 4. Group C was administered Mao-to from day 2 to day 6. Group D was administered Mao-to from day 5 to day 10. Treated mice were followed for survival rates during 2 weeks after infection. Body weight (BW) and organ weights including heart (HW), lungs, thymus and spleen were examined on days 4, 6 and 14. Survival rate of group C (36.4%) was significantly improved compared with group A, B or D (0% of each, P < 0.05). HW and HW/BW ratio in group C was significantly (P < 0.05) lower than those in group A, B or D. Viral titers of hearts were significantly different among groups A, B and C. Cardiac expression in tumor necrosis factor-α (TNF-α) was significantly reduced in group C in comparison with group A, B or D on day 6 by immunohistochemical study. Administration of Mao-to starting on day 2 improves mortality resulting from viral myocarditis in mice with reduced expression of cardiac TNF-α. These findings suggest that timing of Mao-to is crucial for preventing cardiac damage in mice with viral myocarditis.
Copyright © 2010 Zhu Shijie et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.