[Retracted] Protective Effects of Rosa damascena and Its Active Constituent on Aβ(25–35)-Induced Neuritic Atrophy
Figure 2
Protective effects of different fractions of chloroform rose extract on Aβ(25–35)-induced atrophies of dendrites and axons. Cortical neurons were cultured for three days and were then treated with (Veh) or without 10 μM Aβ(25–35) (Cont). Cells were simultaneously treated by the chloroform extract of Rosa damascena (5 μg/mL, RE), subfractions of the chloroform extract (5 μg/mL), NGF (100 ng/mL) or vehicle (0.1% DMSO, Veh). Five days after treatment, the cells were fixed and immunostained for MAP2a&2b (a) or phosphorylated NF-H (b). The lengths of MAP2a&2b-positive neurites or phosphorylated NF-H-positive were measured. Values are means ± SE of data from four to six areas. *P < 0.05 versus Aβ(25–35)/Veh (Student's t-test). Typical photographs were shown.
