Research Article
Antiapoptotic and Antioxidant Properties of Orthosiphon stamineus Benth (Cat's Whiskers): Intervention in the Bcl-2-Mediated Apoptotic Pathway
Table 2
Effects of extract/fractions of O. stamineus on cell viability.
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*Cell viability after 2 h exposure in 400 μmol/L H2O2 was assayed by MTT. Cells were preincubated with different tested samples (62.5, 125, 250, and 500 μg/mL) for 10 min before the addition of 400 μmol/L H2O2. After 2 h incubation, MTT was added to a final concentration of 0.5 mg/mL and then incubated at 37°C for another 2 hrs; the resultant formazan product was extracted for 4 h with DMSO and detected by UV-spectrometer at 570 nm. Values are expressed as means ± SD (). *, ** versus hydrogen peroxide control. |