Rottlerin Inhibits Lonicera japonica-Induced Photokilling in Human Lung Cancer Cells through Cytoskeleton-Related Signaling Cascade
Figure 2
Effects of rottlerin on the photoactivated Lonicera japonica extract-induced cell death and changes in cell morphology. Cells were pretreated with 10 μM rottlerin (RO) for 1 h and then treated with 0.1% ethanol or 100 μg/mL Lonicera japonica extracts (LJ) for 4 h and 0.8 J/cm2 light dose. (a) After irradiation, the viable cells were measured by MTT assay. Results are expressed as the mean percentage of control ± S.D. † compared to the control values. * compared to the photoactivated Lonicera japonica extract-treated cells. (b) Morphological analysis by phase-contrast microscopy of CH27 cells. After irradiation, the cells were immediately photographed. (i) control cells; (ii) Lonicera japonica-treated cells; (iii) rottlerin-treated cells; (iv) cells were pretreated with rottlerin and then Lonicera japonica. All results are representative of three independent experiments.
