Purification and the Secondary Structure of Fucoidanase from Fusarium sp. LD8
Figure 5
Effects of temperature on fucoidanase activity (a) and stability. (b) Effects of temperature on fucoidanase activity was obtained by the following method: adding 0.1 mL of enzyme solution to 1.0 mL of substrate solution (citric acid-citric sodium buffer, pH 6.0), then incubating for 10 min at different temperatures from 30°C to 80°C, respectively. Temperature on stability of the fucoidanase was determined by assaying the residual activity under the following method: 0.1 mL of enzyme solution was incubated at 30 to 80°C for 1 h, rapidly cooled in an ice bath for 5 min, and then removed to 25°C. When the sample reached 25°C, 1.0 mL of substrate solution was added, and the residual enzyme activity was determined and expressed relative to the maximum activity.
