Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2011 (2011), Article ID 196190, 8 pages
http://dx.doi.org/10.1155/2011/196190
Research Article

Purification and the Secondary Structure of Fucoidanase from Fusarium sp. LD8

Department of Biological and Environmental Sciences, Hefei University, Hefei 230022, China

Received 15 January 2011; Revised 25 April 2011; Accepted 25 July 2011

Copyright © 2011 Wu Qianqian et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. D. Anastyuk, N. M. Shevchenko, E. L. Nazarenko et al., “Structural analysis of a highly sulfated fucan from the brown alga Laminaria cichorioides by tandem MALDI and ESI mass spectrometry,” Carbohydrate Research, vol. 345, no. 15, pp. 2206–2207, 2010. View at Publisher · View at Google Scholar · View at Scopus
  2. J. Wang, Q. Zhang, Z. Zhang, H. Zhang, and X. Niu, “Structural studies on a novel fucogalactan sulfate extracted from the brown seaweed Laminaria japonica,” International Journal of Biological Macromolecules, vol. 47, no. 2, pp. 126–131, 2010. View at Publisher · View at Google Scholar · View at Scopus
  3. M. I. Bilan, A. A. Grachev, A. S. Shashkov, N. E. Nifantiev, and A. I. Usov, “Structure of a fucoidan from the brown seaweed Fucus serratus L,” Carbohydrate Research, vol. 341, no. 2, pp. 238–245, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. S. D. Anastyuk, N. M. Shevchenko, E. L. Nazarenko, P. S. Dmitrenok, and T. N. Zvyagintseva, “Structural analysis of a fucoidan from the brown alga Fucus evanescens by MALDI-TOF and tandem ESI mass spectrometry,” Carbohydrate Research, vol. 344, no. 6, pp. 779–787, 2009. View at Publisher · View at Google Scholar · View at Scopus
  5. M. I. Bilan, A. A. Grachev, N. E. Ustuzhanina, A. S. Shashkov, N. E. Nifantiev, and A. I. Usov, “A highly regular fraction of a fucoidan from the brown seaweed Fucus distichus L,” Carbohydrate Research, vol. 339, no. 3, pp. 511–517, 2004. View at Publisher · View at Google Scholar · View at Scopus
  6. I. R. L. Albuquerque, K. C. S. Queiroz, L. G. Alves, E. A. Santos, E. L. Leite, and H. A. O. Rocha, “Heterofucans from Dictyota menstrualis have anticoagulant activity,” Brazilian Journal of Medical and Biological Research, vol. 37, no. 2, pp. 167–171, 2004. View at Google Scholar · View at Scopus
  7. P. A. S. Mourão, “Use of sulfated fucans as anticoagulant and antithrombotic agents: future perspectives,” Current Pharmaceutical Design, vol. 10, no. 9, pp. 967–981, 2004. View at Publisher · View at Google Scholar · View at Scopus
  8. W. J. Kim, Y. K. Koo, M. K. Jung et al., “Anticoagulating activities of low-molecular weight fuco-oligosaccharides prepared by enzymatic digestion of fucoidan from the sporophyll of Korean Undaria pinnatifida,” Archives of Pharmacal Research, vol. 33, no. 1, pp. 125–131, 2010. View at Publisher · View at Google Scholar · View at Scopus
  9. T. Nishino, T. Yamauchi, M. Horie, T. Nagumo, and H. Suzuki, “Effects of a fucoidan on the activation of plasminogen by u-PA and t-PA,” Thrombosis Research, vol. 99, no. 6, pp. 623–634, 2000. View at Publisher · View at Google Scholar
  10. D. J. Schaeffer and V. S. Krylov, “Anti-HIV activity of extracts and compounds from algae and cyanobacteria,” Ecotoxicology and Environmental Safety, vol. 45, no. 3, pp. 208–227, 2000. View at Publisher · View at Google Scholar · View at Scopus
  11. H. Maruyama, H. Tamauchi, M. Hashimoto, and T. Nakano, “Antitumor activity and immune response of Mekabu fucoidan extracted from Sporophyll of Undaria pinnatifida,” In Vivo, vol. 17, no. 3, pp. 245–249, 2003. View at Google Scholar · View at Scopus
  12. T. Nishino, C. Nishioka, H. Ura, and T. Nagumo, “Isolation and partial characterization of a novel amino sugar-containing fucan sulfate from commercial Fucus vesiculosus fucoidan,” Carbohydrate Research, vol. 255, pp. 213–224, 1994. View at Publisher · View at Google Scholar · View at Scopus
  13. L. Chevolot, A. Foucault, F. Chaubet et al., “Further data on the structure of brown seaweed fucans: relationships with anticoagulant activity,” Carbohydrate Research, vol. 319, no. 1–4, pp. 154–165, 1999. View at Publisher · View at Google Scholar · View at Scopus
  14. T. Fujikawa, K. Koyabu, and M. Wada, “Enzymes in hepatopancreas of abalone active on fucoidan(1), Crude enzyme and unabsorbed fraction on CM-cellulose,” Nippon Nogeikagaku Kaishi, vol. 53, pp. 87–95, 1979 (Japanese). View at Google Scholar
  15. K. Kitamura, M. Masaru, and T. Yasui, “Enzymic degradation of fucoidan by fucoidanase from the hepatopancreas of Patinopecten yessoensis,” Bioscience, Biotechnology, and Biochemistry, vol. 56, pp. 490–494, 1992. View at Google Scholar
  16. W. Yaphe and K. Morgan, “Enzymic hydrolysis of fucoidin by Pseudomonas atlantica and pseudomonas carrageenovora,” Nature, vol. 183, no. 4663, pp. 761–762, 1959. View at Publisher · View at Google Scholar · View at Scopus
  17. S. Furukawa, T Fujikawa, D. Koga, and A. Ide, “Production of fucoidan-degrading enzymes, fucoidanase, and fucoidan sulfatase by Vibrio sp. N-5,” Nippon Suisan Gakkaishi, vol. 58, pp. 1499–1503, 1992 (Japanese). View at Google Scholar
  18. I. Y. Bakunina, O. I. Nedashkovskaya, S. A. Alekseeva et al., “Degradation of fucoidan by the marine proteobacterium Pseudoalteromonas citrea,” Mikrobiologiya, vol. 71, pp. 41–47, 2002. View at Google Scholar
  19. T. Sakai, H. Kimura, and I. Kato, “A marine strain of Flavobacteriaceae utilizes brown seaweed fucoidan,” Marine Biotechnology, vol. 4, no. 4, pp. 399–405, 2002. View at Publisher · View at Google Scholar · View at Scopus
  20. T. Sakai, K. Ishizuka, and I. Kato, “Isolation and characterization of a fucoidan-degrading marine bacterium,” Marine Biotechnology, vol. 5, no. 5, pp. 409–416, 2003. View at Publisher · View at Google Scholar · View at Scopus
  21. T. Sakai, K. Ishizuka, K. Shimanaka, K. Ikai, and I. Kato, “Structures of oligosaccharides derived from Cladosiphon okamuranus fucoidan by digestion with marine bacterial enzymes,” Marine Biotechnology, vol. 5, no. 6, pp. 536–544, 2003. View at Publisher · View at Google Scholar · View at Scopus
  22. T. Sakai, H. Kimura, and I. Kato, “Purification of sulfated fucoglucuronomannan lyase from bacterial strain of Fucobacter marina and study of appropriate conditions for its enzyme digestion,” Marine Biotechnology, vol. 5, no. 4, pp. 380–387, 2003. View at Publisher · View at Google Scholar · View at Scopus
  23. P. Wang, J. Cai, S. Qin et al., “Fermentation of marine bacterium Bacillus sp. H-TP2 for fucoidanase and enzyme properties,” Food and Fermentation Industry, vol. 30, pp. 13–15, 2004 (Chinese). View at Google Scholar
  24. A. M. Urvantseva, I. Y. Bakunina, O. I. Nedashkovskaya, S. B. Kim, and T. N. Zvyagintseva, “Distribution of intracellular fucoidan hydrolases among marine bacteria of the family Flavobacteriaceae,” Applied Biochemistry and Microbiology, vol. 42, no. 5, pp. 484–491, 2006. View at Publisher · View at Google Scholar · View at Scopus
  25. V. Descamps, S. Colin, M. Lahaye et al., “Isolation and culture of a marine bacterium degrading the sulfated fucans from marine brown algae,” Marine Biotechnology, vol. 8, no. 1, pp. 27–39, 2006. View at Publisher · View at Google Scholar · View at Scopus
  26. K. Wu, Q. Wu, J. Yan et al., “Production of fucoidanase from the marine fungus Dendryphiella arenaria TM94 by solid substrate fermentation,” ESMB, vol. 5, pp. 12–14, 2002. View at Google Scholar
  27. Q. Wu, J. Cai, K. Wu, and S. Qin, “Solid state fermentation of fucoidanase of marine fungus LD8. Marine Sciences,” Marine Sciences, vol. 28, pp. 23–27, 2004 (Chinese). View at Google Scholar
  28. R. M. R. Jasso, C. N. A. Gonzalez, L. Pastrana, and J. A. Teixeira, “Identification and evaluation of fungal strains with fucoidan degradation potential,” in Proceedings of the 10th International Chemical and Biological Engineering Conference (CHEMPOR '08), pp. 2106–2109, Braga, Portugal, September 2008.
  29. T. Sakai, T. Kawai, and I. Kato, “Isolation and characterization of a fucoidan-degrading marine bacterial strain and its fucoidanase,” Marine Biotechnology, vol. 6, no. 4, pp. 335–346, 2004. View at Publisher · View at Google Scholar · View at Scopus
  30. M. M. Bradford, “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding,” Analytical Biochemistry, vol. 72, no. 1-2, pp. 248–254, 1976. View at Google Scholar · View at Scopus
  31. G. L. Miller, “Use of dinitrosalicylic acid reagent for determination of reducing sugar,” Analytical Chemistry, vol. 31, no. 3, pp. 426–428, 1959. View at Google Scholar · View at Scopus
  32. H. Lineweaver and D. Burk, “The determination of enzyme dissociation constants,” Journal of the American Chemical Society, vol. 56, no. 3, pp. 658–666, 1934. View at Google Scholar · View at Scopus
  33. S. Furukawa, T. Fujikawa, D. Koga, and A. Ide, “Purification and some properties of exo-type fucoidanase from Vibrio sp. N-5,” Bioscience, Biotechnology, and Biochemistry, vol. 56, pp. 1829–1834, 1992. View at Google Scholar
  34. R. Daniel, O. Berteau, J. Jozefonvicz, and N. Goasdoue, “Degradation of algal (Ascophyllum nodosum) fucoidan by an enzymatic activity contained in digestive glands of the marine mollusc Pecten maximus,” Carbohydrate Research, vol. 322, no. 3-4, pp. 291–297, 1999. View at Publisher · View at Google Scholar · View at Scopus
  35. Q. Wu, M. Zhang, K. Wu, B. Liu, J. Cai, and R. Pan, “Purification and characteristics of fucoidanase obtained from Dendryphiella arenaria TM94,” Journal of Applied Phycology, vol. 23, no. 2, pp. 197–203, 2011. View at Publisher · View at Google Scholar
  36. S. Ma, The studies on the preparation, enzymatic properties and characterization of fucoidanase from marine Fusarium, M.S. thesis, Anhui Agriculture University, China, 2006.
  37. S. Ma, H. Xiao, Q. Wu, R. Pan, and J. Cai, “Investigation of fucoidanase by FTIR spectra,” Spectroscopy and Spectral Analysis, vol. 28, pp. 590–593, 2010 (Chinese). View at Google Scholar