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Evidence-Based Complementary and Alternative Medicine
Volume 2011, Article ID 209406, 5 pages
Research Article

Isolation and Identification of Acholeplasma sp. from the Mud Crab, Scylla serrata

1Municipal Key Laboratory of Microorganism and Environmental Engineering, Ningbo, Zhejiang 315100, China
2College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang 315100, China
3College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China

Received 5 January 2011; Accepted 3 June 2011

Copyright © 2011 Ji-Gang Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


For the first time, a mollicute-like organism (MLO) was cultured from moribund mud crabs (Scylla serrata) during an outbreak of clearwater disease in Zhejiang Province, China. The MLO displayed a fried-egg colony morphology in culture, did not possess a cell wall, and was not retained by 0.45 μm and 0.2 μm filters. It was able to ferment glucose, sucrose, lactose, and maltose, but it did not utilize arginine and urea. The MLO grew in the absence of bovine serum and was not susceptible to digitonin. Sequence analysis of the 16S rRNA gene revealed that this MLO had 99% identity with Acholeplasma laidlawii PG-8A, which indicates that the organism isolated from mud crabs is a member of the genus Acholeplasma.