Protective Mechanism of KIOM-4 in Streptozotocin-Induced Pancreatic β-Cells Damage Is Involved in the Inhibition of Endoplasmic Reticulum Stress
Figure 4
The effects of KIOM-4 on cell death induced by STZ in CHOP-downregulated cells. (a) Cells were transfected with CHOP siRNA or control siRNA, the transfected cells were treated with KIOM-4 at 50 g/mL. After 1 hour, 10 mM of STZ was added and further incubated for 24 hours. (a) Apoptotic body formation was observed under a fluorescence microscope and quantitated after Hoechst 33342 staining (original magnification x400). Arrows indicate apoptotic bodies. *Significantly different between STZ-treated siControl and STZ-treated siCHOP () and **significantly different between KIOM-4 plus STZ-treated siControl and KIOM-4 plus STZ-treated siCHOP cells (). (b) DNA fragmentation was quantified with an ELISA kit. Measurements were made in triplicate and values are expressed as means SEM. *Significantly different between STZ-treated siControl and STZ-treated siCHOP (), and **significantly different between KIOM-4 plus STZ-treated siControl and KIOM-4 plus STZ-treated siCHOP cells ().
