Original Article

Water Extract of Ashwagandha Leaves Limits Proliferation and Migration, and Induces Differentiation in Glioma Cells

Figure 5

GFAP expression in response to ASH-WEX treatment. (a) Immunofluorescence detection of glial differentiation marker, GFAP, is shown in control, ASH-WEX (0.5 and 1.0%)-treated C6 glioma cultures. Images were captured using Nikon E600 fluorescent microscope. The relative intensity measurement of immunofluorescence is shown (b). Compared with control, significant changes were found in ASH-WEX-treated cells as seen by (b) (P < .02) and (d) (P < .001). (c) Representative western blot hybridization signals for GFAP from control and test samples (0.5 and 1.0% ASH-WEX-treated). (d) Single cell micrographs of GFAP immunofluorescence in control (i–iii) and 0.5% ASH-WEX-treated cultures (iv–vi).