Free Radical Scavenging, Cytotoxic and Hemolytic Activities from Leaves of Acacia nilotica (L.) Wild. ex. Delile subsp. indica (Benth.) Brenan

Figure 3

Antioxidant and anticancer potential of EA extract obtained from leaves of A. nilotica in different assays. Ethanol extract rich in phenolic compounds exhibit free radical scavenging activity, which was confirmed in total antioxidant activity (1) (Reduction of Molybdenum VI thereby confirming electron or hydrogen donating ability), and in 𝛽-carotene bleaching assay (2) (Zone of color retention indicating their free radical scavenging ability). These two activities are responsible for prevention of ROS. Ethanol extract exhibit antitumor activity, which was confirmed in MTT assay (3) (less cell viability indicating their cytotoxic effect) and hemolytic activity (4) (no lysis indicating that cytotoxic effects is not due to lytic property of the membrane).