Carthamus tinctorius Enhances the Antitumor Activity of Dendritic Cell Vaccines via Polarization toward Th1 Cytokines and Increase of Cytotoxic T Lymphocytes
Figure 3
CT extract enhances the maturation of CD117+-derived DCs in the presence of tumor antigen. Mouse bone marrow cells were collected as described in
Section 2. After 6 days of differentiation with GM-CSF and IL-4, the cells were pulsed with JC tumor
lysate (1.7 × 106 per 0.2 mL PBS; DC + JC) and treated with various concentrations of CT extract
(5, 10, and 20 μg/mL; DC + JC + CT-5, DC + JC + CT-10 and DC + JC + CT-20) in the absence of GM-CSF and IL-4 for
additional 48 h. The medium was collected, and the concentrations of secreted cytokines were determined.
Data from triplicate points are expressed as the mean ± SE. Statistical analysis was performed by one-way ANOVA followed by
Dunnett's test. A
was considered to reflect a significant difference.
* ,
**,
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