Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells

Figure 1

Alignment of deduced amino acid sequence of articulatin B-chain (GeneBank accession no. EF620539), ML-I B-chain (A58957), and ricin D B-chain (P02879). Residues participating in N- and C-terminal sugar binding sites of the B-chain are indicated by ▲ [9, 10]; ● denotes highly conserved residues forming a hydrophobic core of B-chain domains. Residues participating in sugar binding sites, but distinct from the conserved residues, are in boldface type. Joined arrows mark Cys residues forming intrachain disulfide bonds. Cys residues forming interchain disulfide bond are marked with ∘. Potential N-glycosylation sites are boxed.